Microorganisms are active in daily life, they are ubiquitous, and have a significant impact on human life. This project hopes to attract the public to provide samples, and establish a relatively complete system through the combination of experiments, theories and software to illustrate some specific human activities. The specific effects of contact, conversation, respiration and other behaviors, as well as the distribution, quantity, time and space of the microbe produced by the microorganisms brought by the skin, hair, clothing and other organisms on the indoor, such as the surface of the object and the indoor air, are studied. The influence of these indoor microorganisms on human health, such as immune system, intestinal system and respiratory system, is also studied. In addition, some related factors will be considered, such as the differences and causes of indoor microbiological groups at different sites, the supplement of microbial pathogenicity files, and the influence of human flow on indoor microbial groups.
项目组人员：
陶昕宇2017级环境科学与工程专业，何星辰2019级环境科学与工程专业，赵旌灼 2017级会计学专业。
项目指导教师：蔡伟伟
推荐级别：北京市级
研究背景：
随着新冠疫情的爆发，人们对室内微生物的种类、致病性和预防措施等相关信息的关注越来越高。然而关于这方面的研究，涵盖范围较小，例如季节、地区和对象等方面均存在残缺。因此，为弥补学术空白，本项目选择威海秋季高校教室固体表面微生物组特征进行研究。
实验过程：
我们选择一栋教学楼的三个教室作为研究对象。第一步：实地采样，第二步：测序分析，第三步：数据处理。
应用：
我们所研究的室内微生物可应用：
1. 获取微生物的基本信息，如生存条件、具体来源、致病性等，为预防医学中病原体的筛查提供充足依据，使采取措施更高效更有针对性。
2. 让一些被人忽视但比较重要的微生物受到重视，从而完善微生物档案网，并向公众普及更多微生物知识，从而保护人体健康。
未来展望：
在现有基础上去构建一个室内微生物体系。
项目创新点：
弥补学术空缺，研究开发较少的领域，从而抛砖引玉，同时也为后续学者提供较为可靠的研究数据和研究方法。
找出不同位点上微生物群落特征的差异性，发现这些位点共有和特有的菌属，并探讨相关现象与人类微生物交互作用的关联。
项目成果：
1、已找出不同位点上微生物组的差异性。
Fig. 1 Alpha Diversity of Different Locations under Chao Index
Fig. 2 Alpha Diversity of Different Locations under Shannon Index
2、已收集相关微生物基本信息，并通过公众号科普。
3、已进行微生物溯源分析。
Table 1
Source Tracking of Desk
Table 2
Source Tracking of Chair
Table 3
Source Tracking of Knob
Table 4
Source Tracking of Tile
